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AVAILABLE PROTOCOLS – please send updates & new protocols to Phil for posting
Anesthesia for gymnotiform fish (Brachyhypopomus) Aquacalm (Metomidate HCl) anesthesia Clove Oil (eugenol) anesthesia
Cell Culture
Electrophysiology Salines_from_molar_solutions.xls Sterno_salines_for_electrocytes.xls
Electric Organ (EO) Electrocyte extraction for mRNA
Endocrinology Short-lasting steroid implant production
EOD recording & playback EOD Machine – use instructions
Fish and pool care Hoagland plant nutrient solution Walter’s solution (no phosphate) Walter’s solution (phosphate buffered – will grow algae)
Histology
Miscellany Molar_calculations.xls useful spreadsheet for making solutions
Molecular biology Cloning voltage gated sodium channels RT-PCR - J. Tackney RT-PCR - V. Salazar
Surgery
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