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Animal Care

Lab Protocols

Undergraduate training

 

 

AVAILABLE PROTOCOLS

– please send updates & new protocols to Phil for posting

 

Anesthesia for gymnotiform fish (Brachyhypopomus)

            Aquacalm (Metomidate HCl) anesthesia

            Clove Oil (eugenol) anesthesia

            Fast-acting MS-222 anesthesia

            2-Phenoxyethanol anesthesia

 

Cell Culture

            Culturing Electrocytes

 

Electrophysiology

            Chloriding Solution

            Hickman’s Ringers

            Saline preparation

            Salines_from_molar_solutions.xls

            Sterno_salines_for_electrocytes.xls

 

Electric Organ (EO)

            Electric organ dissection

Electrocyte dissociation

Electrocyte extraction for mRNA

 

Endocrinology

            Short-lasting steroid implant production

            Blood collection

            EIA / ELISA

            Implant weight calculator

            Plasma aloquats

 

EOD recording & playback

            EOD Machine – use instructions

IR LED array fabrication

Rasnow’s calibration dipole

 

Fish and pool care

            Aquarium room care

            Alphanumeric tagging

            Fish breeding

Fish tub & pool care

            Hoagland plant nutrient solution

            Hydrogen peroxide solution

            Parasite treatment

            Roof care guide

            Sample SOP for fish

Stress Coat

Walter’s solution (no phosphate)

Walter’s solution (phosphate buffered – will grow algae)

 

Histology

            Cryosectioning tips

            Reagent handling

            Vibrotome sectioning

 

Miscellany

            DrieRite

            Molar_calculations.xls            useful spreadsheet for making solutions

            Saline solutions & buffers

 

Molecular biology

            Chemical solutions

Cloning

Cloning voltage gated sodium channels

Genome sizing

MCR tree instructions

RNAlater

RT-PCR - J. Tackney

RT-PCR - V. Salazar

Quant RT-PCR

Trizol

 

Surgery

            Surgical knots